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An integrated microfluidic system for bovine DNA purification and digital PCR detection
Authors:Qingchang Tian  Ying Mu  Yanan Xu  Qi Song  Bingwen Yu  Congcong Ma  Wei Jin  Qinhan Jin
Affiliation:1. Research Center for Analytical Instrumentation, Institute of CyberSystems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, Zhejiang, PR China;2. College of Life Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, PR China
Abstract:In this paper, we described an integrated modularized microfluidic system that contained two distinct functional modules, one for nucleic acids (NA) extraction and the other for digital PCR (dPCR), allowing for detecting the bovine DNA in ovine tissue.
Keywords:Digital PCR   Integrated microfluidic system   DNA purification   Polydimethylsiloxane
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