DNA biosensor based on hybridization refractory mutation system approach for single mismatch detection |
| |
Authors: | Hamdi Joda Valerio Beni Ioanis Katakis Ciara K O’Sullivan |
| |
Institution: | 1. INTERFIBIO Research Group, Departament d’Enginyeria Quimica, Universitat Rovira i Virgili, 43007 Tarragona, Spain;2. Institucio Catalana de Recerca i Estudis Avançats, Passeig Lluis Companys 23, 08010 Barcelona, Spain |
| |
Abstract: | We report on a simple approach to enhance solid-phase hybridization-based single base mismatch discrimination at high ionic strength based on the deliberate insertion of a natural DNA base mismatch in the surface-tethered probe. A large drop in hybridization signal of single base mismatched alleles using the designed probe as compared with the conventional probe, from 80% to less than 25% of the signal obtained with the fully complementary, non-mutation-containing sequence, when using colorimetric detection was further improved to 20% when using electrochemical detection, attributable to a difference of spacing of immobilized probes. Finally, the designed probe was used for the electrochemical detection of the DQA1*05:05 allele amplified from real human blood samples. |
| |
Keywords: | Hybridization refractory mutation system Single base mismatch Electrochemical genosensor DQA1*05:05 allele |
本文献已被 ScienceDirect 等数据库收录! |
|