A precise spectrophotometric method for measuring sodium dodecyl sulfate concentration |
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Authors: | Kevin R. Rupprecht Ewa Z. LangSvetoslava D. Gregory Janet M. BergsmaTracey D. Rae Jeffrey R. Fishpaugh |
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Affiliation: | Diagnostics Analytical Chemistry Research, Abbott Laboratories, Abbott Park, IL 60049, USA |
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Abstract: | Sodium dodecyl sulfate (SDS) is used to denature and solubilize proteins, especially membrane and other hydrophobic proteins. A quantitative method to determine the concentration of SDS using the dye Stains-All is known. However, this method lacks the accuracy and reproducibility necessary for use with protein solutions where SDS concentration is a critical factor, so we modified this method after examining multiple parameters (solvent, pH, buffers, and light exposure). The improved method is simple to implement, robust, accurate, and (most important) precise. |
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Keywords: | Sodium dodecyl sulfate (SDS) Concentration Quantitative analysis |
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