High-performance liquid chromatography separation of the (S,S)- and (R,S)-forms of S-adenosyl-l-methionine |
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| Authors: | Jianyu Zhang Judith P Klinman |
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| Institution: | 1. Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA;2. California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, CA 94720, USA;3. Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA |
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| Abstract: | S-adenosyl-l-methionine (AdoMet), an important biological cofactor, exists in two chiral forms, (S,S)- and (R,S)-, only the former of which is biologically active. Here, we have developed a chromatographic method to obtain pure (S,S)-AdoMet using a single C18 column. |
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| Keywords: | S-adenosyl-l-methionine Chiral resolution Diastereoisomer High-performance liquid chromatography Radioactive Separation |
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