Detection of 3-phenoxybenzoic acid in river water with a colloidal gold-based lateral flow immunoassay |
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Authors: | Yuan Liu Aihua Wu Jing Hu Manman Lin Mengtang Wen Xiao Zhang Chongxin Xu Xiaodan Hu Jianfeng Zhong Lingxia Jiao Yajing Xie Cunzhen Zhang Xiangyang Yu Ying Liang Xianjin Liu |
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Institution: | 1. College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;2. Key Laboratory of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China |
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Abstract: | 3-Phenoxybenzoic acid (3-PBA) is a general metabolite of synthetic pyrethroids. It could be used as a generic biomarker for multiple pyrethroids exposure for human or pyrethroid residues in the environment. In this study, monoclonal antibodies (mAbs) against 3-PBA were developed by using PBA–bovine serum albumin (BSA) as an immunogen. In the competitive enzyme-linked immunosorbent assay (ELISA) format, the I50 and I10 values of purified mAbs were 0.63 and 0.13 μg/ml, respectively, with a dynamic range between 0.19 and 2.04 μg/ml. Then, the colloidal gold (CG)-based lateral flow immunoassay was established based on the mAbs. The working concentration of coating antigen and CG-labeled antibodies and the blocking effects were investigated to get optimal assay performance. The cutoff value for the assay was 1 μg/ml 3-PBA, and the detection time was within 10 min. A total of 40 river water samples were spiked with 3-PBA at different levels and determined by the lateral flow immunoassay without any sample pretreatments. The negative false rate was 2.5%, and no positive false results were observed at these levels. This lateral flow immunoassay has the potential to be an on-site screening method for monitoring 3-PBA or pyrethroid residues in environmental samples. |
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Keywords: | 3-Phenoxybenzoic acid Lateral flow immunoassay Monoclonal antibodies Colloidal gold |
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