Competitive binding of EF1 and EF2 by mammalian ribosomes: role of GTP hydrolysis in overcoming inhibition by EF2 of aminoacyl-tRNA binding |
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| Authors: | B S Baliga M G Schechtman H N Munro |
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| Affiliation: | Physiological Chemistry Laboratories Department of Nutrition and Food Science Massachusetts Institute of Technology, Cambridge, Mass. 02139 USA |
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| Abstract: | Preincubation of mammalian ribosomes with EF2 retards subsequent binding of EF1-leucyl tRNA when GTP hydrolysis does not occur. This indicates that both the elongation factors have a common binding site on the ribosome. Since the conditions permitting hydrolysis of GTP overcome this inhibition, the possible regulatory role of GTP at the common site on the ribosome is discussed. |
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