水稻零等位RFLP标记的遗传学研究

采用8种限制性内切酶与247个已定位到水稻12条染色体上的分子探针组合,对典型籼稻(Oryza sativa L.ssp.indica)“南京11”和粳稻(O.sativa L.ssp.japonica)“金南凤M”以及中间型品种“Bellemont”之间存在的零等位位点进行了分析,9个探针揭示出零等位位点的存在。其中,RG573、G122、G93、G402和G1318特异揭示了“南京11”的零等位,G261特异揭示了“金南凤M”的零等位,RG229特异揭示了“Bellemont”的零等位,C397揭示了“南京11”和“Bellemont”的零等位,而RG131揭示了“金南凤M”和“Bellemont”的零等位。利用“南京11”/“金南凤M”的杂交F_2群体,对其中58个单株进行了7个零等位RFLP标记及程氏指数法中的6个形态性状的测验,据此计算出各植株的零等位基因型及籼、粳分类指数,结果表明:零等位标记在F_2群体中呈显性遗传。在F_2群体中进一步对G93、G122、G261、G402、G1318、C397、RG573零等位标记与程氏指数之间进行了成组数据t测验,发现G93、G122、G402、G1318、G397差异达显著或极显著水平,G261、RG573的t值也较大,显示出零等位标记在籼、粳交后代,可特异鉴定籼、粳差异。

GENETIC ANALYSIS OF NULL ALLELIC RFLP MARKERS IN RICE

Using 247 RFLP markers distributed on 12 chromosomes combined with 8 kinds of restriction enzymes, null allelism were analyzed in " Nanjing 11" ( Oryza saliva L. ssp. indica) , "Kinmaze ge mutant" ( O. saliva L. ssp. japonica ) and " Bellemont" (intermediate type ) . Nine probes showed null allelism, among which RG573, G93, G122, G402 and G1318 were specific for "Nanjing 11",G261 for "Kinmaze ge mutant", RG229 for " Bellemont", C397 for both "Nanjing 11" and "Bellemont", and RG131 for "Bellemont" and "Kinmaze ge mutant". The null allelic genotype and the indica or japonica phenotype of each individual in the segregation population ("Nanjing 11 "/Kinmaze ge mutant) was described by using 7 null allelic RFLP markers and morphological index characters. The null allelic RFLP markers displayed dominant inheritance in the F2 population, T-test showed that the t-values of G93,G122,G1318 and G397 were extremely significant, and the t-values of G261 and RG573 were also quite significant, indicating that null allelic probes could be used for identifying indica and japonica rice specifically.