| 查尔酮异构酶基因的克隆序列分析及在大肠杆菌中的表达 |
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| 引用本文： | 颜华 李翊云. 查尔酮异构酶基因的克隆序列分析及在大肠杆菌中的表达[J]. Acta Botanica Sinica, 1997, 39(11): 1030-1034 |
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| 作者姓名： | 颜华 李翊云 |
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| 作者单位： | 北京大学生命科学学院蛋白质工程及植物基因工程国家重 |
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| 摘 要： | 从矮牵牛(Petunia hybrida Vilm.)花瓣的cDNA中克隆了查尔酮异构酶(chalcone isomerase,CHI)的基因chi-a,进行了序列分析。结果表明,chi-a基因全长726 bp,编码241个氨基酸。并在大肠杆菌中表达了chi-a基因,对来源于不同植物种的CHI进行了同源性比较分析。
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| 关 键 词： | 查尔酮异构酶 矮牵牛 序列分析 表达 同源性比较 |
MOLECULAR CLONING, SEQUENCING OF CHALCONE ISOMERASE GENE AND ITS EXPRESSION IN E. COLI |
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| Yan Hua,Song Yun,Li Yi-yun,Yan Rui-ming,Li Yi and Chen Zhang-liang. MOLECULAR CLONING, SEQUENCING OF CHALCONE ISOMERASE GENE AND ITS EXPRESSION IN E. COLI[J]. , 1997, 39(11): 1030-1034 |
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| Authors: | Yan Hua Song Yun Li Yi-yun Yan Rui-ming Li Yi Chen Zhang-liang |
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| Abstract: | Anthocyanidins belong to a class of biosynthetic metabolites of flavonoids in higher plants. Chalcone isomerase (CHI) catalyzes the conversion of naringenin chalcone into flavanone. Chi-a gene was cloned from Petunia hybrida Vilm. The sequence of the coding region was 726 bp, encoding a protein of 241 amino acids and was expressed in E. coli. The amino acid sequence ho-mology was compared with CHI from a variety with plant species. |
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| Keywords: | Chalcone isomerase Petunia hybrida Sequence analysis Expression Homology comparison |
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