| 青枯菌细胞表面的疏水性 |
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| 引用本文： | 林娟?,曾金晶,刘树滔,饶平凡.青枯菌细胞表面的疏水性[J].微生物学通报,2011,38(1):45-50. |
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| 作者姓名： | 林娟? 曾金晶 刘树滔 饶平凡 |
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| 作者单位： | 福州大学生物科学与工程学院,福建,福州,350108 |
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| 基金项目： | 福建省自然科学基金项目(No. 2008J0066); 福建省高等学校新世纪优秀人才支持计划项目(No. XSJRC2007-09); 福州大学人才基金资助项目 |
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| 摘 要： | 分别采用BATH和HIC方法测定青枯菌细胞表面疏水性(CSH), 并比较菌液与正十二烷比例(BATH方法)和菌液上样量(HIC方法)对CSH测定结果的影响。确定在BATH方法中菌液(OD600=0.5)与正十二烷的比例为2:1, HIC方法中菌液(OD600=1.0)上样量为0.2 mL; 在此条件下, BATH和HIC两种方法之间呈现出良好的线性关系(r=0.99)。进一步采用HIC方法测定青枯菌在生长过程中CSH的变化情况, 结果显示随培养时间的延长, CSH逐渐降低, 24 h后CSH趋于稳定, CSH与青枯菌细胞表面的EPSⅠ (胞外酸性多糖)含量呈负相关。3株不同致病强度的青枯菌的试验结果进一步验证了青枯菌细胞表面的CSH随EPSⅠ含量的增加而降低。
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| 关 键 词： | 青枯菌 细胞表面 疏水性 EPSⅠ含量 |
| 收稿时间： | 2010/8/19 0:00:00 |
| 修稿时间： | 2010/11/17 0:00:00 |
Cell surface hydrophobicity of Ralstonia solanacearum |
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| LIN Juan,ZENG Jin-Jing,LIU Shu-Tao and RAO Ping-Fan.Cell surface hydrophobicity of Ralstonia solanacearum[J].Microbiology,2011,38(1):45-50. |
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| Authors: | LIN Juan ZENG Jin-Jing LIU Shu-Tao and RAO Ping-Fan |
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| Institution: | College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian 350108, China;College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian 350108, China;College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian 350108, China;College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian 350108, China |
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| Abstract: | Bacterial adhesion to hydrocarbons(BATH) and hydrophobic interaction chromatography(HIC) were employed to determine cell surface hydrophobicity(CSH) of Ralstonia solanacearum,respectively.The effects of the ratio of bacterial suspension to n-dodecane in the BATH assays and the injection volume of bacterial suspension in the HIC assays on the measurement of CSH were investigated.The optimal conditions for CSH analysis were as follow: the ratio of bacterial suspension(OD600=0.5) to n-dodecane was 2:1 and the ... |
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