Farseer-NMR: automatic treatment,analysis and plotting of large,multi-variable NMR data |
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Authors: | João M C Teixeira " target="_blank">Simon P Skinner " target="_blank">Miguel Arbesú Alexander L Breeze " target="_blank">Miquel Pons |
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Institution: | 1.Department for NMR-based Structural Biology,Max-Planck Institute for Biophysical Chemistry,G?ttingen,Germany;2.NMR & Structural Chemistry Division,CSIR-Indian Institute of Chemical Technology,Hyderabad,India;3.Department of Medicine, James Graham Brown Cancer Center,University of Louisville,Louisville,USA |
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Abstract: | Molecular dynamics play a significant role in how molecules perform their function. A critical method that provides information on dynamics, at the atomic level, is NMR-based relaxation dispersion (RD) experiments. RD experiments have been utilized for understanding multiple biological processes occurring at micro-to-millisecond time, such as enzyme catalysis, molecular recognition, ligand binding and protein folding. Here, we applied the recently developed high-power RD concept to the Carr–Purcell–Meiboom–Gill sequence (extreme CPMG; E-CPMG) for the simultaneous detection of fast and slow dynamics. Using a fast folding protein, gpW, we have shown that previously inaccessible kinetics can be accessed with the improved precision and efficiency of the measurement by using this experiment. |
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