Control of bacteriophage mu lysogenic repression |
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Authors: | Ranquet Caroline Toussaint Ariane de Jong Hidde Maenhaut-Michel Geneviève Geiselmann Johannes |
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Institution: | Laboratoire du Contr?le de l'Expression Génique, Institut Jean Roget-Faculté de Médecine-Pharmacie, Domaine de la Merci, F-38700 La Tronche, France. cranquet@cea.fr |
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Abstract: | The transposable and temperate phage Mu infects Escherichia coli where it can enter the lytic life-cycle or reside as a repressed and integrated prophage. The repressor protein Rep is the key element in the lysis-lysogeny decision. We have analyzed the fate of Rep in different mutants by Western blotting under two conditions that can induce a lysogen: high temperature and stationary phase. We show that, unexpectedly, Rep accumulates under all conditions where the prophage is completely derepressed, and that this accumulation is ClpX-dependent. An analysis of the degradation kinetics shows that Rep is a target of two protease systems: inactivation of either the clpP or lon gene results in a stabilization of Rep. Such a reaction scheme explains the counterintuitive observation that derepression is correlated with high repressor concentration. We conclude that under all conditions of phage induction the repressor is sequestered in a non-active form. A quantitative simulation accounts for our experimental data. It provides a model that captures the essential features of Mu induction and explains some of the mechanisms by which the physiological signals affecting the lysis-lysogeny decision converge onto Rep. |
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Keywords: | phage Mu lysis-lysogeny decision modeling repressor proteases |
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