A new,robust, and nonradioactive approach for exploring N-myristoylation |
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| Authors: | Francesca Rampoldi Roger Sandhoff Robert W. Owen Hermann-Josef Gr?ne Stefan Porubsky |
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| Affiliation: | *Department of Cellular and Molecular Pathology, German Cancer Research Center, Heidelberg, Germany;†Lipid Pathobiochemistry Group, German Cancer Research Center, Heidelberg, Germany;§Division of Preventive Oncology, German Cancer Research Center, Heidelberg, Germany; and;**Institute of Pathology, University Medical Center Mannheim, University of Heidelberg, Mannheim, Germany |
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| Abstract: | Myristoyl-CoA (CoA):protein N-myristoyltransferase (NMT) catalyzes protein modification through covalent attachment of a C14 fatty acid (myristic acid) to the N-terminal glycine of proteins, thus promoting protein-protein and protein-membrane interactions. NMT is essential for the viability of numerous human pathogens and is also up-regulated in several tumors. Here we describe a new, nonradioactive, ELISA-based method for measuring NMT activity. After the NMT-catalyzed reaction between a FLAG-tagged peptide and azido-dodecanoyl-CoA (analog of myristoyl-CoA), the resulting azido-dodecanoyl-peptide-FLAG was coupled to phosphine-biotin by Staudinger ligation, captured by plate-bound anti-FLAG antibodies and detected by streptavidin-peroxidase. The assay was validated with negative controls (including inhibitors), corroborated by HPLC analysis, and demonstrated to function with fresh or frozen tissues. Recombinant murine NMT1 and NMT2 were characterized using this new method. This versatile assay is applicable for exploring recombinant NMTs with regard to their activity, substrate specificity, and possible inhibitors as well as for measuring NMT-activity in tissues. |
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| Keywords: | azido-fatty acid N-myristoyl transferase protein acylation Staudinger ligation |
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