The Temperature Dependent Proteomic Analysis of Thermotoga maritima
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Authors: | Zhuowei Wang Wei Tong Quanhui Wang Xue Bai Zhen Chen Jingjing Zhao Ningzhi Xu Siqi Liu |
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Affiliation: | 1. Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, People''s Republic of China.; 2. The Department of Medicine, University of Louisville, Louisville, Kentucky, United States of America.; Lawrence Berkeley National Laboratory, United States of America, |
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Abstract: | Thermotoga maritima (T. maritima) is a typical thermophile, and its proteome response to environmental temperature changes has yet to be explored. This study aims to uncover the temperature-dependent proteins of T. maritima using comparative proteomic approach. T. maritima was cultured under four temperatures, 60°C, 70°C, 80°C and 90°C, and the bacterial proteins were extracted and electrophoresed in two-dimensional mode. After analysis of gel images, a total of 224 spots, either cytoplasm or membrane, were defined as temperature-dependent. Of these spots, 75 unique bacterial proteins were identified using MALDI TOF/TOF MS. As is well known, the chaperone proteins such as heat shock protein 60 and elongation factor Tu, were up-regulated in abundance due to increased temperature. However, several temperature-dependent proteins of T. maritima responded very differently when compared to responses of the thermophile T. tengcongensis. Intriguingly, a number of proteins involved in central carbohydrate metabolism were significantly up-regulated at higher temperature. Their corresponding mRNA levels were elevated accordingly. The increase in abundance of several key enzymes indicates that a number of central carbohydrate metabolism pathways of T. maritima are activated at higher temperatures. |
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