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Micronutrient status and global DNA methylation in school-age children
Authors:Wei Perng  Laura S. Rozek  Mercedes Mora-Plazas  Ofra Duchin  Constanza Marin  Yibby Forero  Ana Baylin  Eduardo Villamor
Affiliation:1.Department of Epidemiology; University of Michigan School of Public Health; Ann Arbor, MI USA;2.Department of Environmental Health Sciences; University of Michigan School of Public Health; Ann Arbor, MI USA;3.Fundación para Investigación en Nutrición y Salud, FINUSAD; Bogotá, Colombia;4.Nutrition Group; National Institute of Health; Bogotá, Colombia
Abstract:Aberrations in global LINE-1 DNA methylation have been related to risk of cancer and cardiovascular disease. Micronutrients including methyl-donors and retinoids are involved in DNA methylation pathways. We investigated associations of micronutrient status and LINE-1 methylation in a cross-sectional study of school-age children from Bogotá, Colombia. Methylation of LINE-1 repetitive elements was quantified in 568 children 5–12 years of age using pyrosequencing technology. We examined the association of LINE-1 methylation with erythrocyte folate, plasma vitamin B12, vitamin A ferritin (an indicator of iron status) and serum zinc concentrations using multivariable linear regression. We also considered associations of LINE-1 methylation with socio-demographic and anthropometric characteristics. Mean (± SD) LINE-1 methylation was 80.25 (± 0.65) percentage of 5-mC (%5-mC). LINE-1 methylation was inversely related to plasma vitamin A. After adjustment for potential confounders, children with retinol levels higher than or equal to 1.05 µmol/L showed 0.19% 5-mC lower LINE-1 methylation than children with retinol levels lower than 0.70 µmol/L. LINE-1 methylation was also inversely associated with C-reactive protein, a marker of chronic inflammation, and female sex. We identified positive associations of maternal body mass index and socioeconomic status with LINE-1 methylation. These associations were not significantly different by sex. Whether modification of these exposures during school-age years leads to changes in global DNA methylation warrants further investigation.
Keywords:C-reactive protein   LINE-1   children   global DNA methylation   inflammation   maternal BMI   methyl-donor nutrients   socioeconomic status   vitamin A
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