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Specific Association of Lectin LecB with the Surface of Pseudomonas aeruginosa: Role of Outer Membrane Protein OprF |
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| Authors: | Horst Funken Kai-Malte Bartels Susanne Wilhelm Melanie Brocker Michael Bott Manjeet Bains Robert E. W. Hancock Frank Rosenau Karl-Erich Jaeger |
| Affiliation: | 1. Institute of Molecular Enzyme Technology, Heinrich-Heine-University Duesseldorf, Juelich, Germany.; 2. Institute of Bio- and Geoscience 1, Forschungszentrum Jülich, Juelich, Germany.; 3. Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada.; 4. Institute of Pharmaceutical Biotechnology, Ulm-University, Ulm, Germany.; Vrije Universiteit Brussel, Belgium, |
| Abstract: | The fucose binding lectin LecB affects biofilm formation and is involved in pathogenicity of Pseudomonas aeruginosa. LecB resides in the outer membrane and can be released specifically by treatment of an outer membrane fraction with fucose suggesting that it binds to specific ligands. Here, we report that LecB binds to the outer membrane protein OprF. In an OprF-deficient P. aeruginosa mutant, LecB is no longer detectable in the membrane but instead in the culture supernatant indicating a specific interaction between LecB and OprF. |
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