| 条斑紫菜中一种琼胶多糖的分离纯化及鉴定 |
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| 引用本文： | 张伟云,陈颢,汪水娟,谭仁祥,费修绠. 条斑紫菜中一种琼胶多糖的分离纯化及鉴定[J]. 植物学通报, 2000, 17(5): 429-434 |
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| 作者姓名： | 张伟云 陈颢 汪水娟 谭仁祥 费修绠 |
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| 作者单位： | [1]南京大学生物科学与技术系,南京 [2]中国科学院海洋研究所,青岛 |
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| 摘 要： | 条斑紫菜（Ｐｏｒｐｈｙｒａ ｙｅｚｏｅｎｓｉｓ）的热水提取物，经ＤＥ－２３和Ｓｅｐｈａｄｅｘ Ｇ－２００柱层析纯化，得到一种含微量硫酸基的琼胶精品（ＰＹ１）。此多糖经Ｓｅｐｈａｒｏｓｅ ６Ｂ柱层析鉴定为单一组分，分子量为２２０ｋＤ。紫外光谱显示它不含蛋白质、多肽及核酸。红外光谱揭示它含３，６－内醚－半乳糖的特征吸收以及微量的硫酸基。该多糖的水解产物经气相色谱分析，主要由半乳糖及其衍生物组成，有少量
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| 关 键 词： | 条斑紫菜 琼胶多糖 分离 纯化 光谱鉴定 |
| 修稿时间： | 1999-07-12 |
TheIsolation, Purification and Identification of a Kind ofAgar Polysaccharide from Porphyra yezoensis |
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| ZHANG Wei_Yun CHEN Hao WANG Shui_Juan TAN Ren_Xiang [WTBZ]. TheIsolation, Purification and Identification of a Kind ofAgar Polysaccharide from Porphyra yezoensis[J]. Chinese Bulletin of Botany, 2000, 17(5): 429-434 |
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| Authors: | ZHANG Wei_Yun CHEN Hao WANG Shui_Juan TAN Ren_Xiang [WTBZ] |
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| Affiliation: | ZHANG Wei_Yun CHEN Hao WANG Shui_Juan TAN Ren_Xiang [WT6BZ] |
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| Abstract: | The crude polysaccharide was extracted from Porphyra yezoensis with hot water and was then purified by DE_23 and Sephadex G_200 column chromatography. A purified polysaccharides (PY1) was obtained, which contains little sulfate. Its homogeneity was identified with Sepharose 6B column chromatography. The average molecular weight was estimated to be 220 kD. The ultraviolet spectrum showed there is no proteins and nucleic acids in PY1. And the infrared spectrum indicated that there are special absorption of 3,6_anhydro_galactose and very little sulfated absorbance peaks. Galactose and its derivatives were the main constituents by gas chromatography. Through periodate oxidation, Smith degradation and 13 C_NMR analysis, PY1 is mainly composed of agarobiose, and a little of its precusor exists as well. |
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| Keywords: | Porphyra yezoensis Polysaccharide Isolation and purification 13 C_NMR |
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