Localization of actin in normal human hepatocytes using fluorescent phallotoxins and immunohistochemical amplification. |
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| Authors: | L Benkoel J Brisse C Capo A M Benoliel P Bongrand T Garcia A Chamlian |
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| Institution: | Laboratoire d'Anatomie Pathologique et de Cytochimie, Faculté de Médecine, Marseille, France. |
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| Abstract: | Two different methods, fluorescent phallotoxins and immunohistochemical amplification systems were used to visualize actin in normal human hepatocytes. With fluorescent phallotoxins (NBD-phallacidin or rhodamine phalloidin), F-actin was distributed along the plasma membranes and at the bile canaliculi. With immunohistochemical methods (biotin-avidin, biotin-streptavidin, silver enhancement), actin was found at the same level, however a cytoplasmic staining was observed and discussed as G-actin localization. |
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