The lipid fluidity of rat liver membrane subfractions |
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Authors: | Whetton A D Houslay M D Dodd N J Evans W H |
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Institution: | Paterson Laboratories, Christie Hospital and Holt Radium Institute, Withington, Manchester M20 9BX, U.K. |
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Abstract: | 1. The lipid fluidity of three major rat liver plasma-membrane subfractions, as well as Golgi apparatus and endocytic fractions, was assessed with a fatty acid spin probe by using e.s.r. techniques. 2. The sinusoidal (blood-facing) plasma-membrane subfraction was the most fluid of the three plasma-membrane regions. Fractions originating from the bile-canalicular and contiguous (lateral) regions were most rigid. Endocytic fractions isolated (endosomes and diacytosomes) were of a similar fluidity to fractions originating from the sinusoidal plasma-membrane region. By far the most fluid fractions examined were derived from the Golgi-apparatus complex. 3. The three plasma-membrane subfractions each showed a different response to the bilayer-fluidizing effect of benzyl alcohol. 4. Arrhenius-type plots of the order parameter S and outer hyperfine splitting, 2T , identified lipid-phase separations in the plasma-membrane subfractions. |
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