Establishment and characterization of endometrial undifferentiated carcinoma cell line (TMCC-2.U) |
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| Authors: | H Iwabuchi H Nakaya H Sakunaga J Nagata M Sakamoto K Okabe Y Negishi M Sugita T Sugishita Y Tenjin |
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| Institution: | Department of Obstetrics and Gynecology, Tokyo Medical College Hospital, Japan. |
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| Abstract: | We established new cell line designed TMCC-2.U, which suggested transformation to undifferentiated carcinoma, derived from endometrial clear cell carcinoma cell line (TMCC-2). The monolayer culture cell showed a pavement arrangement and spindle like shape. A rough-endoplasmic reticulum, mitochondria etc. are well developed. But cytoplasmic endocrine granulosa were so poorly, it suggests functional developments are poor. The TMCC-2.U cells were transplanted to nude mice which showed no typical pattern suggested undifferentiated carcinoma. Their chromosome number varied and the mode is 78. Marker chromosome were found frequency. Growth pattern and production of tumor marker are clearly differentiate from TMCC-2. As mensioned above, TMCC-2.U cell line will be very valuable in basic research on mechanism of transformation and effects of patient's serum on hystogenesity. |
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