Sensitivity of Partially Purified Ice Nucleation Activity of Fusarium acuminatum SRSF 616 |
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Authors: | Tricia L Humphreys Louela A Castrillo Marcia R Lee |
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Institution: | (1) Department of Microbiology, Miami University, Oxford, OH 45056, USA, US;(2) Department of Zoology, Miami University, Oxford, OH 45056, USA, US |
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Abstract: | Factors that affect bacterial ice nucleation, including growth medium, growth phase, nutrient deprivation, and cold-temperature exposure, were investigated in the ice nucleation active (INA) fungus Fusarium acuminatum SRSF 616. Ice nucleation activity remained relatively constant throughout the growth cycle, and the cell-free culture supernatant consistently displayed higher ice nucleation activity than the hyphal pellet. Although nutrient starvation and low-temperature exposure enhance bacterial ice nucleation activity, reducing the concentration of C, N, or P in synthetischer nährstoffarmer broth (SNB) did not increase fungal ice nucleation activity, nor did exposure to 4°C or 15°C. From the SNB supernatant, selected INA chromatography fractions were obtained that demonstrated increased sensitivity to proteinase K and heat compared with culture supernatant. We propose that partial purification of the fungal ice nuclei resulted in removal of low-molecular-weight stabilizing factors. |
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