The use of indole as a spectrophotometric assay substrate for toluene dioxygenase |
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Authors: | Richard O. Jenkins Howard Dalton |
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Affiliation: | Department of Biological Sciences, University of Warwick, Coventry, CV7 7AL, U.K. |
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Abstract: | Abstract Cell extracts of toluene-grown Pseudomonas putida produced a soluble yellow dye during aerobic incubations with indole and NADH. Accumulation of indoxyl in reaction mixtures corresponded with a linear increase in absorbance at 400 nm. The rate of increase in absorbance was shown to be a specific measure of toluene dioxygenase activity. The primary product of toluene oxidation, cis -toluene dihydrodiol, inhibited dioxygenase activity in cell extracts containing no detectable activity of cis -toluene dihydrodiol dehydrogenase. |
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Keywords: | Pseudomonas putida cell extracts indoxyl cis-toluene dihydrodiol |
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