Sensitivity of OR in Phage λ |
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Authors: | Audun Bakk Ralf Metzler and Kim Sneppen |
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Institution: | NORDITA (Nordic Institute for Theoretical Physics), DK-2100 Copenhagen, Denmark |
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Abstract: | We investigate the sensitivity of the right operator in bacteriophage lambda. In particular, the system is probed in the three different regulatory protein concentration-regimes: 1), lysogen (CI dominates); 2), during induction (CI and Cro at comparable concentrations); and 3), after induction (Cro dominates). Systematic perturbations of the protein-operator binding energies show in a lysogen that the activity (production rate) at promoter PRM is robust to variations, in contrast to PR, where the sensitivity is high. Both promoters, however, show large sensitivity in regimes 2 and 3. In all regimes we identify several suppressors, meaning that for a given large perturbation (±2 kcal/mol) of one binding energy, there exist compensating perturbation(s) that restore the wild-type activity. |
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