Myo-Inositol Turnover in the Intact Rat Brain: Increased Production after d-Amphetamine |
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Authors: | Amiram I Barkai |
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Institution: | New York State Psychiatric Institute and Department of Psychiatry, Columbia University College of Physicians and Surgeons, New York, New York, U.S.A. |
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Abstract: | Abstract: Apparent turnover of myo -inositol in the brain of urethane-anesthetized rats was estimated in vivo from the rate of appearance of endogenous myo -inositol in the cerebroventricular compartment. Ventricular-cisternal perfusion technique combined with isotope dilution of 14C] myo -inositol was used to determine the rate of appearance of brain-produced myo -inositol and its modification by d -amphetamine. A mean value of 0.75 nmol/min was obtained for the rate of appearance in the cerebroventricular system. A dose dependent increase in this rate was seen after the administration of d -ampheta-mine. The endogenous removal of myo -inositol from the perfusate was also studied and found to be mediated in part by a saturable transport system that was not influenced by d-amphetamine. The rate of entry of myo -inositol from blood to the erebroventricular system was very low and accounted for only 2% of the total rate of appearance, indicating that the majority of myo -inositol in the rat cerebroventricular fluid originates in the brain. |
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Keywords: | Cerebral myo-inositol Turnover rate Cerebroventricular perfusion Saturable transport d-Amphetamine |
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