Determination of position of substitution on 2-acetamido-2-deoxy-D-galactosyl residues in glycolipids. |
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| Authors: | S K Kundu R W Ledeen P A Gorin |
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| Institution: | Albert Einstein College of Medicine of Yeshiva University, The Saul R. Korey Department of Neurology and the Department of Biochemistry, 1300 Morris Park Avenue, Bronx, New York 10461 U.S.A.;Prairie Regional Laboratory, National Research Council of Canada, Saskatoon, Saskachewan Canada |
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| Abstract: | The substitution site on 2-acetamido-2-deoxy-D-galactosyl residues in oligosaccharide chains of glycolipids was determined by permethylation of the glycolipid with methyl iodide in the presence of dimethylsulfinyl carbanion, methanolysis of the permethylated product under mild conditions, acetylation with acetic anhydride-pyridine, and identification of the resulting substituted methyl glycosides of 2-deoxy-2(N-methylacetamido)-D-galactose by g.l.c. The method was applied to glycolipids of known structure, including normal brain ganglioside, Tay-Sachs ganglioside, and Forssman glycolipid. |
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| Keywords: | To whom inquiries should be sent |
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