Effects of alloxan and streptozotocin on calcium transport in isolated mouse liver mitochondria |
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Authors: | L Nelson L Boquist |
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Institution: | Institute of Pathology, University of Umeå, S-901 87 Umeå, Sweden |
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Abstract: | The effect of alloxan and streptozotocin on the fluxes of Ca2+ in isolated mouse liver mitochondria was studied with dual wave-length spectrophotometry, using antipyrylazo III as metallochromic indicator. Streptozotocin had no effect on Ca2+ uptake, whereas alloxan inhibited the initial rate and extent of Ca2+ influx in a way dependent on the duration of preincubation, and occurrence of Pi in the reaction mixture. A rapid release of Ca2+ followed upon addition of either FCCP or alloxan after the reaction had been started. When added to preloaded mitochondria, alloxan induced a concentration dependent release of Ca2+. The data suggest that alloxan induces an initial release of mitochondrial Ca2+, which is followed by inhibition of Ca2+ influx. The initial release may be due to uncoupler activity induced by alloxan, and the inhibition of Ca2+ influx may be a consequence of inhibited Pi transport. |
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Keywords: | Antipyrylazo III bis(4-antipyrylazo)-4 5-dihydroxy-2 7-naphthalene-disulphonic acid EDTA ethylenediaminetetraacetate FCCP p-trifluoromethoxycarbonylcyanidephenylhydrazone NEM N-ethylmaleimīde |
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