Two classes of glutathione S-transferase genes with different response profiles to bacterial challenge in Venerupis philippinarum |
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Authors: | Li Chenghua Su Xiurong Li Ye Li Taiwu Sun Chongjie Zhou Tingting Liu Haipeng |
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Institution: | a Faculty of Life Science and Biotechnology, Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang Province 315211, PR China b Ningbo City College of Vocational Technology, Ningbo 315100, PR China c State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, PR China |
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Abstract: | Glutathione S-transferase (GST) is major cytosolic detoxification enzymes involved in many pathological and physiological processes. In the present study, two classes of GSTs (VpGST-1 and VpGST-2) were cloned from Venerupis philippinarum haemocytes by cDNA library and RACE approaches. Sequence alignments and phylogenetic analysis together supported that they belonged to a new member of sigma and pi classes GSTs protein family, respectively. The expression profiles of these two genes under Vibrio anguillarum challenge were investigated by quantitative RT-PCR. The bacterial challenge could significantly up-regulate the mRNA expression of both VpGST-1 and VpGST-2 with larger amplitude in VpGST-2, and the feedback speed for VpGST-2 was more rapid than that of VpGST-1. The differences in the response to bacterial challenge indicated that they were functional diversity and probably played cooperative roles in mediating the Vibrio challenge in clam. |
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Keywords: | Venerupis philippinarum Glutathione S-transferase Expression profile Vibrio anguillarum |
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