CFTR mediated chloride secretion in the avian renal proximal tubule |
| |
Authors: | Laverty Gary Anttila Ashley Carty Jenava Reddy Varudhini Yum Jamie Arnason Sighvatur S |
| |
Institution: | a Department of Biological Sciences, University of Delaware, Newark, DE 19716, USAb Department of Physiology, University of Iceland, IS-101 Reykjavík, Iceland |
| |
Abstract: | In primary cell cultures of the avian (Gallus gallus) renal proximal tubule parathyroid hormone and cAMP activation generate a Cl−-dependent short circuit current (ISC) response, consistent with net transepithelial Cl− secretion. In this study we investigated the expression and physiological function of the Na-K-2Cl (NKCC) transporter and CFTR chloride channel, both associated with Cl− secretion in a variety of tissues, in these proximal tubule cells. Using both RT-PCR and immunoblotting approaches, we showed that NKCC and CFTR are expressed, both in proximal tubule primary cultures and in a proximal tubule fraction of non-cultured (native tissue) fragments. We also used electrophysiological methods to assess the functional contribution of NKCC and CFTR to forskolin-activated ISC responses in filter grown cultured monolayers. Bumetanide (10 μM), a specific blocker of NKCC, inhibited forskolin activated ISC by about 40%, suggesting that basolateral uptake of Cl− is partially mediated by NKCC transport. In monolayers permeabilized on the basolateral side with nystatin, forskolin activated an apical Cl− conductance, manifested as bidirectional diffusion currents in the presence of oppositely directed Cl− gradients. Under these conditions the apical conductance appeared to show some bias towards apical-to-basolateral Cl− current. Two selective CFTR blockers, CFTR Inhibitor 172 and GlyH-101 (both at 20 μM) inhibited the forskolin activated diffusion currents by 38-68%, with GlyH-101 having a greater effect. These data support the conclusion that avian renal proximal tubules utilize an apical CFTR Cl− channel to mediate cAMP-activated Cl− secretion. |
| |
Keywords: | Avian proximal tubule Bumetanide Cystic fibrosis transmembrane regulator (CFTR) CFTR Inhibitor 172 Forskolin GlyH-101 Short circuit current Sodium-potassium-2 chloride transporter (NKCC) |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|