Entwicklung eines hochempfindlichen Enzymimmuntests zum Nachweis von Ochratoxin A |
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Authors: | Schneider E Usleber E Dietrich R Märtlbauer E |
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Institution: | 1.Institut für Tier?rztliche Nahrungsmittelkunde,Justus-Liebig-Universit?t,Gie?en;2.Lehrstuhl für Hygiene und Technologie der Milch,Ludwig-Maximilians-Universit?t,München |
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Abstract: | Antibodies against ochratoxin A were produced in rabbits after immunization with an ochratoxin A-keyhole limpet hemocyanine conjugate. The immunogen was found to be very efficient, and high antibody titers were detected in the sera of all immunized rabbits. In a competitive enzyme immunoassay using ochratoxin A-horseradish peroxidase as the labelled antigen, low levels of ochratoxin A in buffer solution could be detected. The mean standard curve detection limit and 50% inhibition level of the optimized assay were at 15 pg/ml and 50 pg/ml, respectively. Relative cross-reactivity of ochratoxin B was found to be 2%. With these characteristics, this novel enzyme immunoassay should be useful for the routine detection of ochratoxin A in food and in biological samples at levels well below 1 ng/g. |
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