Bovine superoxide dismutase: a radioimmunoassay. |
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| Authors: | K Kelly C Barefoot A Sehon A Petkau |
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| Affiliation: | 1. Department of Immunology, Faculty of Medicine, University of Manitoba, Basic Medical Sciences Building, 730 William Avenue, Winnipeg, Manitoba, Canada, R3E OW3;2. Medical Biophysics Branch, Whiteshell Nuclear Research Establishment, Atomic Energy of Canada Limited, Pinawa, Manitoba, Canada, ROE 1LO |
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| Abstract: | Rabbit antibodies to bovine superoxide dismutase have been produced and used to develop a double-antibody solid phase radioimmunoassay for the enzyme. The assay is sensitive and highly specific for the bovine enzyme, showing no cross-reactivity with the murine or human superoxide dismutases. It has been applied to the quantitation of exogenous enzyme in serum and extracts of mouse cells and tissues. |
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| Keywords: | To whom correspondence should be addressed. |
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