In vitro synthesis of infectious retroviral RNA. |
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| Authors: | F Flamant and J A Sorge |
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| Institution: | Department of Basic and Clinical Research, Research Institute of Scripps Clinic, La Jolla, California 92037. |
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| Abstract: | A plasmid was constructed in which a T7 RNA polymerase promoter was placed upstream of a recombinant amphotropic retrovirus genome containing a selectable neomycin resistance gene. To test the infectivity of the RNA produced by T7 RNA polymerase in vitro, the RNA was microinjected into the nuclei of psi 2 packaging cells. Infectious particles conferring G418 resistance were released. |
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