| 微卫星PCR产物变性与非变性PAGE-银染检测方法的比较 |
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| 引用本文： | 曲鲁江,李显耀,杜志强,张龙超,杨宁.微卫星PCR产物变性与非变性PAGE-银染检测方法的比较[J].遗传,2004,26(4):522-524. |
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| 作者姓名： | 曲鲁江 李显耀 杜志强 张龙超 杨宁 |
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| 作者单位： | 中国农业大学动物科技学院，北京 100094 College of Animal Science and Technology, China Agricultural University, Beijing, 100094 China |
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| 基金项目： | 国家杰出青年科学基金(30225032)~~ |
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| 摘 要： | 用鸡的微卫星引物对6个中国地方鸡种的两个微卫星位点进行PCR扩增。将扩增产物在变性与非变性聚丙烯酰胺凝胶（PAGE）上进行电泳，经银染，表明微卫星产物在二者上的电泳结果有明显的差异。在非变性聚丙烯酰胺凝胶中，表现为有较多的非特异带，而在变性胶中微卫星扩增产物条带清晰，易于鉴定。Abstract：The genomic DNAs from six chicken breeds in China were amplified using two microsatellite primers. The PCR products were detected by non-denatured and denatured PAGE gels respectively, and the gels were dyed by silver. There were distinct differences between the two kinds of gel. In non-denatured gels. There were many nonspecific bands while clear purposed bands were showed in denatured gels.
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| 关 键 词： | 微卫星 PAGE PCR 银染 Key words microsatellite |
| 文章编号： | 0253-9772(2004)04-0522-03 |
| 修稿时间： | 2003年5月30日 |
The Comparision of Denatured and non-denatured PAGE-Silver- Staining Detection of PCR Product of Microsatellites |
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| QU Lu-Jiang,LI Xian-Yao,DU Zhi-Qiang,ZHANG Long-Chao,YANG Ning.The Comparision of Denatured and non-denatured PAGE-Silver- Staining Detection of PCR Product of Microsatellites[J].Hereditas,2004,26(4):522-524. |
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| Authors: | QU Lu-Jiang LI Xian-Yao DU Zhi-Qiang ZHANG Long-Chao YANG Ning |
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| Institution: | College of Animal Science and Technology, China Agricultural University, Beijing 100 094, China. |
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| Abstract: | The genomic DNAs from six chicken breeds in China were amplified using two microsatellite primers. The PCR products were detected by non-denatured and denatured PAGE gels respectively, and the gels were dyed by silver. There were distinct differences between the two kinds of gel. In non-denatured gels. There were many nonspecific bands while clear purposed bands were showed in denatured gels. |
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| Keywords: | microsatellite PAGE PCR silver-staining |
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