In Vivo Quantitative Assessment of Myocardial Structure,Function, Perfusion and Viability Using Cardiac Micro-computed Tomography |
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Authors: | Elza van Deel Yanto Ridwan J. Nicole van Vliet Sasha Belenkov Jeroen Essers |
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Affiliation: | 1.Department of Genetics, Erasmus MC, Rotterdam;2.Department of Experimental Cardiology, Erasmus MC, Rotterdam;3.PerkinElmer, Inc.;4.Department of Vascular Surgery, Erasmus MC, Rotterdam;5.Department of Radiation Oncology, Erasmus MC, Rotterdam |
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Abstract: | The use of Micro-Computed Tomography (MicroCT) for in vivo studies of small animals as models of human disease has risen tremendously due to the fact that MicroCT provides quantitative high-resolution three-dimensional (3D) anatomical data non-destructively and longitudinally. Most importantly, with the development of a novel preclinical iodinated contrast agent called eXIA160, functional and metabolic assessment of the heart became possible. However, prior to the advent of commercial MicroCT scanners equipped with X-ray flat-panel detector technology and easy-to-use cardio-respiratory gating, preclinical studies of cardiovascular disease (CVD) in small animals required a MicroCT technologist with advanced skills, and thus were impractical for widespread implementation. The goal of this work is to provide a practical guide to the use of the high-speed Quantum FX MicroCT system for comprehensive determination of myocardial global and regional function along with assessment of myocardial perfusion, metabolism and viability in healthy mice and in a cardiac ischemia mouse model induced by permanent occlusion of the left anterior descending coronary artery (LAD). |
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Keywords: | Bioengineering Issue 108 Imaging mouse cardiac functional imaging LV function cardiovascular function perfusion imaging myocardial infarction ischemia LAD artery occlusion MicroCT Quantum FX contrast agent eXIA160 |
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