Methods for analyzing peptides and proteins on a chromatographic timescale by electron-transfer dissociation mass spectrometry |
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| Authors: | Udeshi Namrata D Compton Philip D Shabanowitz Jeffrey Hunt Donald F Rose Kristie L |
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| Affiliation: | University of Virginia, Department of Chemistry, McCormick Road, Charlottesville, Virginia 22904, USA. |
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| Abstract: | Advancement in proteomics research relies on the development of new, innovative tools for identifying and characterizing proteins. Here, we describe a protocol for analyzing peptides and proteins on a chromatographic timescale by coupling nanoflow reverse-phase (RP) liquid chromatography (LC) to electron-transfer dissociation (ETD) mass spectrometry. For this protocol, proteins can be proteolytically digested before ETD analysis, although digestion is not necessary for all applications. Proteins
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