Methods for the isolation, sterilization and cultivation of Lemnaceae |
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| Authors: | DAVID W. BOWKER ANTHONY N. DUFFIELD ARICK DENNY |
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| Affiliation: | Department of Applied Biology, The University of Wales Institute of Science and Technology, Cardiff, and Department of Botany and Biochemistry, Westfield College, London, England |
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| Abstract: | SUMMARY. A method is described for isolating and sterilizing fronds of Lemnà gibba, L. minor, L. trisulca, L. polyrrhiza and Wolffia arrhiza , employing enrichment followed by sodium hypochlorite treatment. The method enables about 4% of the total number of treated fronds to be used as initiators of clonal cultures in which bacteria responding to organic enrichment and algae are excluded. Appropriate growth media, culture vessels and culture conditions are detailed. |
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