Proteomic analysis of liver tissues subjected to early ischemia/reperfusion injury during human orthotopic liver transplantation |
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Authors: | Vascotto Carlo Cesaratto Laura D'Ambrosio Chiara Scaloni Andrea Avellini Claudio Paron Igor Baccarani Umberto Adani Gian Luigi Tiribelli Claudio Quadrifoglio Franco Tell Gianluca |
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Institution: | Department of Biomedical Sciences and Technologies, University of Udine, Udine, Italy. |
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Abstract: | Knowledge of early molecular events occurring upon ischemia/reperfusion (I/R) during liver transplantation (LT) is of great importance to improve the therapeutic intervention of surgical treatment. However, nowadays, few data are available on early protein targets of I/R injury. To identify these proteins, we used a differential proteomics approach in the characterization human liver biopsies during I/R upon LT. Analyses were performed on nine donor livers during LT. By using 2-DE and MALDI-TOF MS, we identified 36 proteins which resulted significantly altered upon I/R injury. The majority of these proteins are functionally involved in lipid and energy metabolism, in different metabolic pathways, in redox signalling and in oxidative-stress response. Our data represent the first global approach in the study of I/R injury in liver. |
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Keywords: | Hepatocytes Liver transplantation Oxidative stress |
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