Characterization of partially purified alcohol dehydrogenase from Rhodococcus sp. strain GK1 |
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| Authors: | Krier,Kreit,& Milliè re |
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| Affiliation: | Laboratoire de Fermentations et de Bioconversions Industrielles, Ecole Nationale Supérieure d'Agronomie et des Industries Alimentaires, Institut National Polytechnique de Lorraine, Vandoeuvre-lès-Nancy, France,;Laboratoire de Microbiologie et de Biologie Moléculaire, Département de Biologie, Facultédes Sciences, Rabat, Morocco |
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| Abstract: | An NAD-dependent secondary alcohol dehydrogenase (ADH) produced by Rhodococcus sp. GK1 was purified about fivefold with a yield of 82% by hydrophobic interaction chromatography. This enzyme reduced monoketones, diketones and α-dicarbonyl compounds ; it oxidized secondary alcohols but not primary alcohols. Optimum pH was 7·0 or 8·5 for reduction or oxidation of substrates, respectively, and optimal temperature for activity was 55 °C. The apparent molecular mass of ADH was about 60 kDa by gel filtration chromatography. |
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