A sterol biosynthetic geneAtCYP51A2 promoter for constitutive and ectopic expression of a transgene in plants |
| |
Authors: | Hyoungseok Lee Hyun Jeong Oh Hyo Min Ahn Chang Jae Oh Jin -Ho Jeong Gyeong Lyong Jeon Chung Sun An Sang -Bong Choi Ho Bang Kim |
| |
Institution: | (1) Department of Plant Science, University of Connecticut, Unit 5082, Storrs, CT 06269, USA;(2) Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Biotechnology Research Center, Southwest University, Chongqing, 400716, China;(3) Faculty of Life Sciences, Hubei University, Wuhan, 430062, China;(4) Department of Cell and Structural Biology, University of Illinois, Urbana, IL 61801, USA; |
| |
Abstract: | Arabidopsis CYP51A2 (AtCYP51A2) mediates the sterol 14α-demethylation step inde novo sterol biosynthesis, and is constitutively and highly expressed in all plant tissues (Kim et al., 2005). We exploited the
molecular features of its expression and the fundamental role of sterol biosynthesis in cells to develop a plant-derived promoter.
Our GUS expression analysis between transgenicArabidopsis lines forAtCYP51A2::GUS and35S::GUS revealed that activity of theAtCYP51A2 promoter was comparable to that of the35S promoter, based on enzymatic activities and protein levels. TheAtCYP51A2 promoter was also constitutively active in transgenic tobacco, indicating that 5′ regulatory elements could be conserved
amongCYP51 promoters in dicot plants. A homologue ofAtCYP51A2 was identified from rape seed, a crop species closely related toArabidopsis. Its constitutive tissue expression pattern implies that the application of thisAtCYP51A2 promoter is possible for that species. Based on these results, we present a new binary vector system with the plant-derivedAtCYP51A2 promoter, which is able to constitutively and ectopically drive a transgene in various dicotyledonous plants.
These two authors are equally contributed to this work. |
| |
Keywords: | Arabidopsis CYP51A2 promoter β -glucuronidase binary vector CaMV 35S promoter constitutive overexpression sterol biosynthesis |
本文献已被 SpringerLink 等数据库收录! |
|