Initial site of cleavage towards oxidized B-chain of insulin by thermophilicStreptomyces alkaline proteinase |
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| Authors: | Eiji Ichishima Masashi Matsue |
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| Institution: | (1) Laboratory of Enzymology and Microbial Chemistry, Tokyo Noko University, Fuchu, 183 Tokyo, Japan |
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| Abstract: | The initial site of cleavage towards the oxidized B-chain of insulin by thermophilicStreptomyces alkaline proteinase (EC 3.4.21.14) was investigated. The most susceptible bond was between Leu15 and Tyr16. Additional cleavage of the bonds Leu11-Val12, Leu17-Val18, and Gln4-His5 were noted. The results suggested that the specificity of bond, cleavage of thermophilicStreptomyces alkaline proteinase is close to that of subtilisin Carlsberg. |
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