Detection of genetic variation among single-spore isolates and identification of genets ofArmillaria ostoyae by AFLP analysis with Texas RedTM-labeled selective primer |
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| Authors: | Kazuhisa Terashima Joo Young Cha Kiyoshi Miura |
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| Affiliation: | (1) Division of Environmental Resources, Graduate School of Agriculture, Hokkaido University, 060-8589 Sapporo, Japan;(2) Wakayama Experimental Forest, Faculty of Agriculture, Hokkaido University, Kozakawa, 649-4563 Wakayama, Japan;(3) Present address: The Tottori Mycological Institute, The Japan Kinoko Research Centre Foundation, 689-1125 Tottori, Japan;(4) Present address: Ishiyama 1-6-16-18, 005-0841 Sapporo, Japan |
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| Abstract: | AFLP (amplified fragment length polymorphism) analysis was applied toArmillaria ostoyae isolates (single-spore isolates and field isolates from the same forest). For detection of AFLP, we have developed a modified method using DNA sequencer with Texas Red-labeled selective primer. In analysis of single-spore isolates, this technique provided large numbers of highly polymorphic DNA markers, which can be used to identify genets. The results suggested that outbreeding might be common inA. ostoyae. |
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| Keywords: | AFLP Armillaria root and butt rot genets population structure |
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