我国霍乱弧菌的脂肪酸分型研究

目的 对脂肪酸分型方法在霍乱弧菌菌株鉴定、菌株相似性分析等方面的应用价值进行评价。方法 选取了分离自我国的两个主要致病血清群的194株霍乱弧菌菌株（1961年以来的El Tor型和1992年以来的O139群），提取脂肪酸，应用MIDI公司的脂肪酸分型系统，进行数据分析。结果 检测的所有菌株都含有的脂肪酸成分有13种。霍乱弧菌的判断符合率为88.6%。二维聚类分析没有成明显可区分的群, O139群霍乱弧菌的脂肪酸组成与O1群的相似，产毒与非产毒霍乱弧菌的脂肪酸成分没有显著差异。结论 脂肪酸分型对弧菌种的快速鉴定有应用价值，对霍乱弧菌的现场分离鉴定有辅助意义，在小样本暴发资料的研究中能够反映菌株之间的亲缘关系，但其对霍乱弧菌种属内的各种特征性菌群不具有鉴别能力。

The Fatty Acid Methyl Ester Profiling of Vibrio cholerae in China

Objective:Evaluation the application value of Fatty acid methyl ester (FAME) method in Vibrio cholerae strain identification and relationship judgment. Methods:194 V. cholerae strains belonging to the two main pathogenic seragroups, El Tor isolated since 1961 and O139 since 1992 in China, were selected and fatty acid methyl ester were extracted. Microbial identification system of MIDI company was used to analyse the data. Results:Thirteen fatty acids were common components contained in all of the tested V. cholerae strains. The coincidence of special judgment was 88.6%. Uhere was no obvious separable group in 2-D plot analysis. The fatty acid compositions of O139 serogroup strains were similar to that of O1 V.cholerae strains. There was no significant difference between the fatty acid compositions of the toxigenic and non-toxigenic strains. Conclusion:FAME method is useful in rapid identification of Vibrionaceae strains and in field isolation of V. cholerae. It can be used to judge the relationships of strains in small outbreaks, but it cannot differentiate the subgroups of V. cholerae.