Comparison Between Two Chromogenic Substrates for Revealing an Immunoperoxidase Reaction of Human Metaphase Chromosomes |
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Authors: | M Marcollet J Morin P Lecher |
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Institution: |
a Laboratoire de Chimie Biologique et Pharmacodynamique, U.E.R. de Pharmacie, Clermont, Ferrand, France |
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Abstract: | To study the binding of an antiadenosine serum to human chromosome DNA, two types of chromogenic reagents were compared. The procedure is as follows: lymphocytes with metaphase chromosomes were spread on slides; some slides were irradiated by UV; all slides were then incubated with antiadenosine rabbit serum and then with antirabbit sheep serum labelled with peroxidase; the latter was revealed in the classical manner by 3,3'-diaminobenzidine (DAB), or, alternatively, by p-phenylenediamine plus pyrocatechol (PPD-PC). The present study shows that the results obtained with PPD-PC were equivalent, if not superior, to those obtained with DAB. In the case of weaker reactions, results with PPD-PC were superior. Furthermore, this reagent has the major advantage of being noncarcinogenic. |
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