Interaction of pollen F-actin with rabbit muscle myosin and its subfragments (HMM,S1) |
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Authors: | X Liu L -F Yen |
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Institution: | (1) College of Biological Sciences, Beijing Agricultural University, 100094 Beijing, People's Republic of China |
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Abstract: | Summary Actin purified from maize pollen grains can be polymerized into F-actin which increased the ATPase activities of proteolytic fragments (HMM, S1) of rabbit muscle myosin. The values of Kapp is 232 M for HMM and 290 M for S1, which are six- and seven-fold higher than those of rabbit muscle F-actin under the same conditions. Pollen actin and rabbit muscle myosin form hybrid actomyosin showing increase in viscosity and turbidity of solution. Viscosity and turbidity of the actomyosin dropped and then increased again with addition of ATP. Polymerized pollen actin can be decorated in vitro with both rabbit muscle HMM and S1 to form an arrowhead-shaped structure like that observed in living plant cells. The results show that pollen actin is similar to muscle actin at a qualitative level. But there are differences between them at a quantitative level.Abbreviations HMM
heavy meromyosin
- S1
myosin subfragment 1
- ATP
adenosine-5-triphosphate |
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Keywords: | Pollen actin Rabbit muscle myosin Heavy meromyosin Myosin subfragment 1 |
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