A Heat Shock Protein Gene, CsHsp45.9, Involved in the Response to Diverse Stresses in Cucumber |
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Authors: | Jianwu Li He Zhang Jianbin Hu Junqing Liu Keke Liu |
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Institution: | Open Key Laboratory of Horticultural Plant Physiology and Genetic Improvement, High School of Henan Province, Henan Provincial Key Laboratory of Fruit Plant and Cucurbit, Horticulture College of Henan Agricultural University, Zhengzhou 450002, Henan, China. lijw555@sohu.com |
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Abstract: | Heat shock proteins (Hsps) are a family of highly conserved proteins present in all organisms. They mediate a range of cytoprotective functions as molecular chaperones and are recently reported to regulate the immune response. Using suppression subtractive hybridization, we isolated and characterized a cucumber cDNA, designated CsHsp45.9, which encodes a putative heat shock protein of 45.9 kDa protein, containing three conserved DnaJ domains belonging to the Type I Hsp40 family. Real-time quantitative RT-PCR analysis revealed that CsHsp45.9 was significantly induced in cucumber leaves inoculated with downy mildew (Pseudoperonospora cubensis) in this incompatible interaction. Gene expression was also strongly up-regulated by various abiotic stresses. CsHsp45.9 was mainly expressed in flowers with a flower-specific, stamen- and pistil-predominant expression pattern. This suggests that CsHsp45.9 harbors broad-spectrum responses to both biotic and abiotic stresses and may play a role in downy mildew resistance in cucumber. |
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