Characterization of secreted recombinant <Emphasis Type="Italic">Toxoplasma gondii</Emphasis> surface antigen 2 (SAG2) heterologously expressed by the yeast <Emphasis Type="Italic">Pichia pastoris</Emphasis> |
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| Authors: | Mun Yik Fong Yee Ling Lau Mohamed Zulqarnain |
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| Institution: | (1) Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Malaysia;(2) School of Arts and Sciences, Monash University Sunway Campus, Bandar Sunway, Selangor, Malaysia;(3) Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia |
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| Abstract: | The surface antigen 2 (SAG2) gene of the protozoan parasite, Toxoplasma gondii, was cloned and extracellularly expressed in the yeast Pichia pastoris. The effectiveness of the secreted recombinant SAG2 (rSAG2-S) as a serodiagnosis reagent was assessed by western blots and
ELISA. In the western blot assay, rSAG2-S reacted with all Toxoplasma-antibody positive human serum samples but not with Toxoplasma-negative samples. In the ELISA, rSAG2-S yielded sensitivity rates ranging from 80% (IgG negative, IgM positive) to 100% (IgG
positive, IgM negative). In vivo experiments showed that serum from mice immunized with rSAG2-S reacted specifically with
the native SAG2 of T. gondii. These mice were protected when challenged with live cells of T. gondii. |
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| Keywords: | Pichia pastoris Recombinant SAG2 antigen Toxoplasma gondii |
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