Formation of 2-hydroxy-4-methylpentanoic acid from l-leucine by Clostridium butyricum |
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| Authors: | Marie-José Butel,Alain Rimbault,Nasser Khelifa,Geneviè ve Campion,Odette Szylit,Francis Rocchiccioli |
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| Affiliation: | Laboratoire de Microbiologie, UnitéMicrobiologie Anaérobie, Facultédes Sciences Pharmaceutiques et Biologiques de Paris, UniversitéRenéDescartes, 4 Avenue de l'Observatoire, F-75270 Paris Cedex 06, France; Unitéd'Ecologie et Physiologie de Système Digestif, Equipe Métabolites Bactériens et Santé, Institut National de la Recherche Agronomique, F-78352 Jouy-en-Josas Cedex, France; Laboratoire de Biochimie, Hôpital Saint-Vincent-de-Paul, 74–82 Avenue Denfert-Rochereau, F-75674 Paris Cedex 14, France |
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| Abstract: | Abstract Five Clostridium butyricum strains of different origin were grown in trypticase-yeast extract-hemin medium with or without d-glucose (TGYH or TYH medium, respectively) and in a synthetic basal medium with d-glucose (BMG medium). 2-Hydroxy-4-methylpentanoic acid was detected by gas chromatography-mass spectrometry (GC-MS) for the five strains whether grown in TGYH or TYH medium (270 or 170 μM, respectively). In BMG medium supplemented with l-leucine (10 mM), the concentration of this metabolite was strongly increased (2.8 mM versus 10 μM in the control). After culture in TGYH or TYH medium supplemented with l-( methyl -2H3)leucine, 2-hydroxy-4-([2H3]methyl)pentanoic acid was detected by GC-MS. This observation demonstrates that C. butyricum is able to convert l-leucine into the corresponding 2-hydroxy acid and opens a new aspect in the study of C. butyricum metabolism. |
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| Keywords: | Clostridium butyricum l-Leucine 2-Hydroxy-4-methylpentanoic acid Stable isotope Gas chromatography-mass spectrometry |
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