D-β-Hydroxybutyrate Prevents MPP+-Induced Neurotoxicity in PC12 Cells |
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Authors: | Baohua Cheng Xinxin Yang Chengchun Chen Danfu Cheng Xudong Xu Xuewen Zhang |
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Institution: | (1) Jining Medical University, Jining, Shandong, People’s Republic of China;(2) Rizhao People’s Hospital, Rizhao, Shandong, People’s Republic of China;(3) Wenzhou Medical College, Wenzhou, Zhejiang, People’s Republic of China |
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Abstract: | Numerous studies show that D-β-Hydroxybutyrate (DβHB) is neuroprotective. The present study was to explore the neuroprotective
effects of DβHB against the cell death and apoptosis induced by 1-methyl-4-phenylpyridinium ion (MPP+) in PC12 cells. PC12
cells were pretreated with DβHB and followed by MPP+ exposure. The cell viability was determined by MTT assay. The morphological
characteristics of apoptosis was observed by Acridine Orange (AO) staining and apoptotic rates were measured by flow cytometer.
The product of lipid peroxidation, malondialdehyde (MDA), was measured using thiobarbituric acid method. The mitochondrial
membrane potential (MMP), intracellular ROS and total glutathione were detected by microplate reader. In PC12 cells, pretreatment
with DβHB significantly reduced MPP+-induced the decrease of cell viability. AO staining and flow cytometric analysis found
DβHB inhibited MPP+-induced apoptosis. The measurement of MDA formation showed that DβHB alleviated lipid peroxidation induced
by MPP+. The loss of MMP induced by MPP+ was preventive by DβHB. The changes of intracellular ROS and total glutathione induced
by MPP+ were reversed by DβHB. DβHB protected PC12 cells against MPP+-induced death and apoptosis. |
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