Storage and conversion of Eclipta alba synseeds and RAPD analysis of the converted plantlets |
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| Authors: | A Ray and S Bhattacharya |
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| Institution: | (1) Department of Botany, Laboratory of Morphogenesis, Banaras Hindu University, Varanasi, 221005, Uttar Pradesh, India;(2) Present address: Department of Life Science, Institute of Plant Biology, National Taiwan University, Taipei, Taiwan;(3) Present address: Department of Botany, Laboratory of Algal Biology, Banaras Hindu University, Varanasi, 221005, Uttar Pradesh, India; |
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| Abstract: | The encapsulated shoot tips and nodal segments of Eclipta alba were stored at 4, 12 and 20 °C under irradiance of 1.5 gmmol m−2 s−1 and high conversion was observed in synseeds stored at 4 °C for 8 weeks. Duration of storage was extended up to 12 weeks
by decreasing sucrose concentration in the alginate matrix from 3 to 1 or 2 % and conversion frequency was 71.2–76.1 %. Synseed-derived
plantlets survived by 100 % in ex vitro conditions. RAPD analysis revealed uniform amplification profile in donor and synseed derived plantlets. |
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