Association of SND1 protein to low density lipid droplets in liver steatosis |
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Authors: | I Garcia-Arcos Y Rueda P González-Kother L Palacios B Ochoa O Fresnedo |
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Institution: | 1.Department of Physiology, Faculty of Medicine and Dentistry,University of the Basque Country,Leioa,Spain;2.Science Faculty,Sma. Concepción Catholic University,Caupolicán,Chile;3.Progenika Biopharma S.A.,Derio,Spain |
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Abstract: | Although the human homologue of SND p102, p100 coactivator, was initially described as a nuclear protein, the p100 coactivator
protein family members have non-nuclear localization in mammalian cells with active lipid handling, storage, and secretion.
However, their role in lipid homeostasis remains unresolved. Here, we investigate the distribution of the rat homologue SND
p102 (also called SND1) and its association with newly formed lipid droplets in the liver parenchyma and cultured hepatocytes.
Sucrose gradient fractionation showed that SND p102 cofractionated with endoplasmic reticulum and Golgi markers. Such cofractionation
was not altered in regenerating steatotic rat liver. However, SND p102 was also detected in lipid droplets from regenerating
liver, showing a specific directionalization to the least dense ones. Confocal microscopy of cultured hepatocytes confirmed
the findings of gradient fractionation. In addition, p100 coactivator was consistently encountered in microsomes and lipid
droplets in control and oleate-treated HepG2 cells. The total amount of SND p102 in hepatocytes was similar in both conditions,
suggesting a specific translocation of the protein. Our findings indicate that SND p102 and the human p100 coactivator have
a ubiquitous cytoplasmic distribution in hepatocytes and that steatogenic conditions promote the targeting of SND p102 from
other cell compartments to specific low density lipid droplets. |
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