Development of a <Emphasis Type="Italic">Bacillus subtilis</Emphasis> expression system using the improved P<Emphasis Type="Italic">glv</Emphasis> promoter |
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Authors: | Yang M Ming Zhang W Wei Chen Y Lin Gong Y Sheng |
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Institution: | (1) College of Animal Science and Technology, Northwest A&F University, 712100 Yangling, China |
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Abstract: | Background
B. subtilis is an important organism in the biotechnological application. The efficient expression system is desirable in production
of recombinant gene products in B. subtilis. Recently, we developed a new inducible expression system in B. subtilis, which directed by B. subtilis maltose utilization operon promoter P
glv
. The system demonstrated high-level expression for target proteins in B. subtilis when induced by maltose. However, the system was markedly repressed by glucose. This limited the application of the system
as a high-expression tool in biotechnology field. The aim of this study was to further improve the P
glv
promoter system and enhance its expression strength. |
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Keywords: | |
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