F0F1-ATPase from Vibrio alginolyticus. Subunit composition and proton pumping activity. |
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| Authors: | A Cuenda F Centeno C Gutierrez-Merino |
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| Institution: | A.N. Belozersky Laboratory for Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR. |
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| Abstract: | An F0F1-ATPase was isolated from the membranes of the marine bacterium Vibrio alginolyticus. Homology between the subunits of the F0-complexes from E. coli and V. alginolyticus was found using antibodies against subunits a, b and c of the E. coli F0F1-ATPase. The F0F1-complex from V. alginolyticus was reconstituted into proteoliposomes, which were competent in ATP-dependent proton uptake. This process was inhibited by triphenyltin, DCCD, and venturicidin. Na+ did not affect proton translocation. |
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